About the Blood Test
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| Blood samples |
Venous blood samples should be taken from the side that is not being infused. If difficult to do, should be taken from the infusion line 5 cc origin who smoked should be removed first. Because the liquid mixture will interfere with serologic reactions. Only later taken without being given 5 cc subsequent anti coagulants sent a blood sample. To reduce hemolysis, blood samples should be taken from a vein easily dipunksi, using needle #22 or #21, and had to suck slowly. (12)
Performed tests ABO blood group system by erythrocytes in tests with antisera against antigenic Anti-A and Anti-B (slide test). In Rh (+) is almost 100%. The above tests should be done at room temperature or cooler (20-22°C). Because at 37°C the reaction becomes weak also be tested against infectious disease agents such as syphilis, HBsAg, anti-HCV. And anti-HIV 1 and 2. (8, 9, 12)
Because almost all of the population has Rh (+). At the very urgent circumstances transfusion if there is not the same blood type, can be used PRC type blood group O. (12)
Major cross-examination, performed by checking serum recipients with donor erythrocytes to detect recipient antibodies that can cause lysis of erythrocytes donor and cause hemolytic transfusion reactions. Minor cross-examination, checking serum donors with recipient’s erythrocytes. Both cross-linking reaction, was done in three phases: 0.9% NaCl medium, albumin, and Coombs, entirely takes 2 hours. (12)
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